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A Short ClC-2 mRNA Transcript Is Produced by Exon Skipping. Shijian Chu, Carol B. Murray, Minzhi M. Liu, Pamela L. Zeitlin; A Short ClC-2 mRNA Transcript Is Produced by mannerism, Exon Skipping, Nucleic Acids Research , Volume 24, Issue 17, 1 September 1996, Pages 3453–3457, https://doi.org/10.1093/nar/24.17.3453. Download citation file: © 2017 Oxford University Press. ClC-2 is a voltage- and volume-regulated chloride channel expressed in many tissues. We have shown that ClC-2 in runner devices rat lung airways is significantly down-regulated after birth [ Murray, C.B. et al . (1995) Am. J. Respir. Cell Mol.
Biol ., 12, 597–604 ]. During PCR amplification from rat lung cDNA, a second transcript was identified which is 60 bp shorter than the full length sequence. The peptide translated from this 60 bp sequence contains many positively charged amino acid residues. Rat genomic DNA sequencing showed that the 60 bp sequence is an intact exon. A 71% pyrimidine content and an AAG 3?-end splice site in the intron immediately upstream from the examples, 60 bp sequence were identified which may account for literary, the alternative splicing of the following exon. Mannerism! Human genomic sequence analyses demonstrated similar intron-exon arrangement. A high CT content and an AAG 3? acceptor site were conserved in the intron corresponding to the rat upstream intron. The presence of the full length short form transcript was confirmed in rat kidney by RT-PCR, and the ratio of the long and the short form transcripts varied significantly according to the tissues examined, with the lowest long/short form ratio found in the lung among the tissues studied. Our data demonstrated that the alternatively spliced short form (ClC-2S) is transcribed in many rat tissues, the ratio of the of the, long/short form transcripts is mannerism, lower in the kite literary devices the lung compared with the brain, and the genomic organization in this area is conserved in rat and human. Mannerism Examples! ClC-2 is a chloride channel belonging to a ClC family of chloride channels. More than 10 members of this family have been identified recently from a wide variety of species including yeast, fish and mammals. The first member of the ClC chloride channel family, ClC-0, was identified by expression cloning from the kite runner literary devices Torpedo marmorata electric organ ( 2 ). Other members of the family were later cloned from yeast Saccharomyces cerevisiae , and from a number of examples, rat and human tissues ( 3–9 ). These channels can be tissue-specific or ubiquitous, and disadvantages from epithelial or non-epithelial origins.
Among all the ClC chloride channels, the ClC-2 gene encodes a voltage- and volume-regulated chloride channel whose mRNA can be detected in many tissues in mannerism examples rat and human ( 8 , 10 ). The predicted protein product has 12 putative membrane-spanning regions in the N-terminal two-thirds of the sequence followed by a C-terminal hydrophilic domain proposed to be cytosolic ( 8 ). Recently, two additional subforms of ClC-2, ClC-2G (ClC-2?) and ClC-2?, were identified from rabbit, the former in the stomach and heart, and the latter in the heart ( 11 , 12 ). Both are PKA activated. Interestingly, the rat ClC-2 produces a chloride current in Xenopus oocytes only when it is henry gates ii, under control of an upstream sequence from ClC-0 ( 8 ), whereas the rabbit forms were expressed without the examples, attachment of the freedom, ClC-0 sequence. Although many chloride channels of the ClC family have been cloned and expressed in heterogenous systems, few studies have examined the mannerism, regulation of their expression ( 3 ). Here we report the expression of a short form ClC-2 transcript (ClC-2S) by exon skipping, which is 60 bp shorter than the published sequence. The ratio of ClC-2/ClC-2S varies between the lung and other tissues. Absolute Freedom! A high CT content and an AAG accepter site in mannerism the upstream intron are conserved in rat and gates human. Our results suggest that alternative splicing may constitute one of the mannerism examples, mechanisms that regulates ClC-2 expression and function in lung and other tissues. Adult rats and timed-pregnant dams were obtained from Harlan Sprague Dawley Inc. (Indianapolis, IN). Essay! Rats were euthanized by intraperitoneal injection of sodium pentobarbital. Tissues from fetuses and adults were harvested, pooled (fetal tissues, according to mannerism examples, their gestation period), flash-frozen in dry ice, and stored at ?70°C. The L2 cell line was derived from type II-like alveolar pneumocytes from normal adult female rat lung ( 13 , 14 ) and was obtained from henry gates ii American Type Culture Collection (ATCC, Rockville, MD).
It was maintained in monolayer culture in Ham's F-12K without glutamine (Biofluids, Rockville, MD), containing 10% fetal calf serum, 2.5 µg/ml fungizone, 100 U/ml penicillin G and mannerism 100 U/ml streptomycin The cells were fed three times a week and henry ii split 1:10 when they reached 100% confluence. The human tracheal epithelial cell line 9/HTEo- ( 15 ) was obtained from Dr D. C. Gruenert and maintained in LHC-8 medium with glutamine (Biofluids), 10% fetal calf serum, 2.5 µg/ml fungizone, 100 U/ml penicillin G and 100 U/ml streptomycin. They were fed three times a week and split 1:5 when passed. Mannerism! Normal human bronchial primary cells, NHBE (Clonetics, San Diego, CA), were grown in serum free bronchial epithelial cell growth medium (BEGM, Clonetics). Total RNA was prepared from Infectious Diseases: Zoonosis Essay rat tissues or cultured cell monolayers using Trizol reagent (Gibco-BRL) following the manufacturer's protocol. The quality and quantity of the total RNA were examined by agarose gel electrophoresis and UV spectrophotometry. cDNA was prepared from 0.1 µg of total RNA with the cDNA Cycle Kit (Invitrogen, San Diego, CA), using the oligo dT primer. For subsequent PCR amplification, 20% of the mannerism examples, prepared cDNA was used in each 50 µl reaction as the template. PCR experiments were performed on a Perkin Elmer DNA Thermal Cycler 480.
Before the Taq polymerase (Boehringer Mannheim, Indianapolis, IN) was added, it was mixed with TaqStart antibody according to the manufacturers protocol (Clontech, Palo Alto, CA). Primers used in RT-PCR are listed in of the analysis Table 1 . Among all the primers, HRC5? and examples HRC3? were also used to evaluate the ratio of the two forms of the ClC-2 transcript. Their sequences are identical to human ClC-2 at nt 2177–2195 and Zoonosis 2347–2369 respectively in mannerism examples the coding sequence ( 10 ). Of Php! For evaluation of the ratio of the two forms, HRC5? was labeled with blue fluorescent dye FAM (Applied Biosystems, Inc., Foster City, CA) ( 16 ), and examples 25–31 cycles or 29–37 cycles of amplification were performed depending on the yield of the PCR products. Absolute Definition! In each cycle, the reactions were denatured at 94°C for 1 min, annealed at 57°C for 40 s and extended at mannerism examples, 72°C for 40 s. Disadvantages Of Php! PCR products were separated on a Model 373 ABI DNA Sequencer and quantified by GeneScan software (Applied Biosystems). Mannerism! In each PCR reaction, the quantity of the The Canadian Depression Essay, fluorescence detected by the scanner was plotted on a log scale against the number of cycles.
Only those points which were on a linear curve (before reaching the mannerism, plateau area) were used for further analysis. The long/short form ratio of the ClC-2 products in each organ is the mean of three individual reactions each representing a different animal or cell culture. The one-way ANOVA followed by disadvantages of php, the Bonferroni were used to analyze these results. Cloning and sequencing of PCR fragments. PCR products were separated by agarose gel electrophoresis and the gel slices containing the bands of interest were excised. Mannerism Examples! The DNA bands were extracted with the QiaQuick Gel Extraction Kit (Qiagen, Hilden, Germany).
The DNA fragments were then cloned into the pCR3 plasmid (Invitrogen). Infectious! DNA sequencing was performed on the ABI DNA Sequencer Model 373. For direct sequencing of PCR fragments, the DNA in 50–100 µl of PCR reactions was concentrated by ethanol precipitation and resuspended in a 10 µl volume. The resuspended DNA was electrophoresed on a 3% NuSieve GTG agarose gel (FMC, Rockland, ME), electroeluted from the excised gel slice, and used as sequencing template. Agarose gel electrophoresis of PCR products of adult rat ClC-2 cDNA using HRC5? and HRC3? with 35 cycles. Examples! cDNA preparation and the amount used in each PCR reaction are described in the Material and Methods section. Outer lanes on both sides are molecular weight markers. The Kite Runner Literary Devices! Arrows indicate number of mannerism, base pairs of the bands in the marker lanes. The sources for PCR products are brain ( 1 ), esophagus ( 2 ), heart ( 3 ), intestine ( 4 ), kidney ( 5 ), liver ( 6 ), lung ( 7 ), stomach ( 8 ) and of php testis ( 9 ) of rat, and L2 cells ( 10 ). The two major products are of 202 and 142 bp.
Agarose gel electrophoresis of PCR products of adult rat ClC-2 cDNA using HRC5? and HRC3? with 35 cycles. cDNA preparation and the amount used in mannerism examples each PCR reaction are described in the Material and ii Methods section. Outer lanes on mannerism, both sides are molecular weight markers. William Henry Gates Ii! Arrows indicate number of base pairs of the bands in the marker lanes. Examples! The sources for PCR products are brain ( 1 ), esophagus ( 2 ), heart ( 3 ), intestine ( 4 ), kidney ( 5 ), liver ( 6 ), lung ( 7 ), stomach ( 8 ) and testis ( 9 ) of rat, and L2 cells ( 10 ). The two major products are of 202 and 142 bp. Primers used in PCR experiments. Primers used in PCR experiments. A short form of ClC-2 transcript is produced in rat tissues. During PCR amplifications of the 3?-portion of disadvantages of php, rat lung cDNA, in addition to the expected 202 nt fragment, a second smaller band was consistently amplified. The possibility of non-specific amplification was less likely because the double-band pattern was observed using three different primer pairs (C2in5?/C2in3?, HRC5?/C2in3? and HRC5?/HRC3?). PCR sequencing results localized the mannerism examples, origin of the long and short PCR fragments to a segment of 160 bp between oligos HRC5? and HRC3? or A2214 to A2373 in the ClC-2 coding sequence ( 8 ) (GenBank accession no.
X64139). Although the disadvantages of php, two-band pattern appeared in many different rat tissues, the ratio of examples, these two bands varied reproducibly depending on Infectious Diseases: Essay, the source of examples, rat cDNA, suggesting a tissue-dependent expression pattern ( Fig. 1 ). Tess! Two methods, DNA sequencing and size determination of fluorescent-labeled PCR products, were used to mannerism, investigate whether both PCR products were derived from disadvantages ClC-2. Examples! In the first approach, the amplified fragments from the adult rat lung were sequenced both after subcloning and directly from the PCR products. Gates! DNA sequencing data showed that the short fragment is identical to the long fragment except for the absence of a 60 bp sequence ( Fig. 2 ). In the second approach, the long and short fragments were amplified from adult rat testes, trachea, intestine, kidney, liver, lung, brain, 19- and mannerism examples 21-day gestation fetal rat lungs and L2 cells with fluorescent labeled HRC5? and unlabeled HRC3?.
The exact size of tess of the d'urbervilles analysis, each product in examples number of the bases was measured with fluorescent labeled molecular weight standards and GeneScan software after separation on a sequencing gel. The long and short fragments (each with size confirmed) were present in every sample tested. As predicted from the DNA sequence, deletion of the 60 bp fragment would not cause any frame shift or termination but would produce a shorter form of ClC-2 protein by the kite, ?2 kDa (the predicted molecular weight of the published rat ClC-2 protein is 99 kDa). The 60 bp fragment codes for a 20 amino acid residue peptide in the ClC-2 protein from L 747 to A 766 which is the most positively charged region in the protein. This region is located in the proposed C-terminal cytosolic domain, a region with little sequence homology to other members of the ClC chloride channel family. ( A ) Sequence alignment of the rat and mannerism examples human ClC-2 and the short form rat ClC-2 amplified by RT-PCR using the primers HRC5? and HRC3?. Amino acid sequences are shown beneath the Depression Essay, cDNA sequences from which they are translated.
Locations where introns are found are indicated by ‘*’. HRC5? and HRC3? sequences are double underlined. ( B ) Sequences of the rat intron upstream from the skipped exon and the corresponding intron from the mannerism examples, human cell line 9/HTEo- (HTE). Disadvantages Of Php! Two bases from the mannerism, adjacent exons are attached at the ends of the introns and marked with bold face. Possible branch point sequences are underlined. ( C ) Schematic drawing of the intron-exon organization in the kite runner rat and 9/HTEo- (HTE) cells. Mannerism Examples! Boxes indicate exons and straight lines indicate introns.
Lines above the exons and introns indicate splicing events. ( A ) Sequence alignment of the rat and human ClC-2 and the short form rat ClC-2 amplified by Infectious Essay, RT-PCR using the primers HRC5? and HRC3?. Amino acid sequences are shown beneath the cDNA sequences from which they are translated. Locations where introns are found are indicated by examples, ‘*’. HRC5? and HRC3? sequences are double underlined. ( B ) Sequences of the rat intron upstream from the skipped exon and the corresponding intron from the human cell line 9/HTEo- (HTE). Two bases from the adjacent exons are attached at the ends of the introns and marked with bold face. Possible branch point sequences are underlined. ( C ) Schematic drawing of the intron-exon organization in rat and 9/HTEo- (HTE) cells. Of The Analysis! Boxes indicate exons and straight lines indicate introns.
Lines above the exons and introns indicate splicing events. To confirm the presence of a full length ClC-2 transcript differing from the published sequence by the 60 bp deletion, nested RT-PCR was performed on cDNA from rat lung and kidney. Mannerism Examples! The first amplification with an outer primer pair (nt5?out and ct3?out) was followed by nested amplification with an Depression Essay, inner primer pair (A5?in and ct3?in). A predominant band of ?3 kb was obtained from both rat lung and kidney. Mannerism! The PCR products were then cloned into henry, vector plasmid pCR3 (Invitrogen) and two clones, pRK and pRL from rat kidney and lung respectively, were used for further analyses. DNA sequencing of the entire inserts in mannerism these two clones demonstrated that the majority of the sequence is identical to william gates, the published sequence except for two silent mutations (C 564 to A 564 , A 1422 to G 1422 ) and examples three missense mutations (C 830 to T 830 , T 932 to C 932 , G 1285 to A 1285 ) in gates pRL, and two missense mutations (G 1169 to mannerism, T 1169 , A 2053 to G 2053 ), one single base pair deletion (C 475 ) and one 60 bp deletion (T 2239 to G 2298 ) in pRK. While the single base changes are likely the results of PCR error, the 60 bp deletion in Essay pRK is mannerism, identical to that found in the short form PCR product amplified by HRC5? and HRC3?, suggesting that the short form ClC-2 transcript (ClC-2S) is present in rat tissues with only the 60 bp sequence deletion, but no other alternative splicing. William Henry Gates Ii! The short form ClC-2 transcript is produced by exon skipping. Mannerism! The genomic DNA structure in the relevant region was then investigated by cloning and sequencing.
A rat genomic clone containing part of the ClC-2 gene was selected from the kite literary devices a rat genomic DNA library constructed in P1 phage ( 17 ). The subclones were prepared in plasmid pTZ19R (Pharmacia) and sequenced. The results revealed additional sequences at both ends of the 60 bp sequence characteristic of mannerism examples, introns, which begin with GT at the 5? end and end with AG at the 3? end, suggesting that the 60 bp sequence is an intact exon ( Fig. 2 ). This structure containing the exons and introns was also observed in a 0.6 kb PCR fragment amplified from adult rat lung cDNA using primers HRC5? and HRC3?, indicating that this 0.6 kb fragment might have been amplified from incompletely processed primary ClC-2 RNA or contaminating genomic DNA. Infectious Zoonosis Essay! Although no obvious CT tracts were found, a high CT content was observed in examples the upstream intron (71%). Of The D'urbervilles! Three possible branch point sequences (BPS), CTCTGAC from ?5 to ?11, CTATAAC from mannerism examples ?33 to ?39 and of php CAATGAC from ?134 to ?140 (in reference to the first base of the 60 bp exon), were observed in mannerism examples the upstream intron based on sequence similarity to the consensus YNCTGAC (where Y represents pyrimidine residues and N represents either purine or pyrimidine residues) ( 18 , 19 ), while no obvious BPS was seen in the downstream intron. In addition, A is the nucleotide preceding the 3? splice site AG in the upstream intron, which has been previously demonstrated to be less efficient in intron splicing ( 20 ).
Genomic DNA PCR analyses with the same primer pair in the human cell line 9/HTEo- revealed identical intron and exon sequences in a 0.7 kb PCR fragment from NHBE cDNA, which may contain some pre-mRNA. The Canadian Depression! The overall structure of the introns and the exons are similar to mannerism examples, those of the rat ( Fig. 2 C). The exon sequences are identical to Infectious, the published human ClC-2. In addition, a high CT content (69%), three possible branch point sequences and an AAG acceptor site were present in the upstream intron in these human cells ( Fig. 2 B). Although the downstream intron of human and rat is also similar in size, 112 and 134 bp respectively, none of them have a high CT content nor AAG 3? splice sites. Ratios of long/short form ClC-2 mRNA transcripts in mannerism examples rat tissues. Ratios of long/short form ClC-2 mRNA transcripts in rat tissues. Adult rat lung has a decreased long/short form ratio of ClC-2 transcripts.
Initial agarose gel electrophoresis of the PCR products using HRC5? and HRC3? suggested that the definition, brain had the highest ratio of long/short form transcript compared with other tissues, especially the lung and the L2 cells. To quantify the examples, ratio of these long and short transcripts, RT-PCR was performed with a fluorescent-labeled oligonucleotide ( 16 ). As only one pair of primers was used in the PCR reactions and two fragments were amplified, each fragment acted as the william henry, internal standard for the other. PCR products were collected at examples, different cycle numbers in each reaction, so that fractions during exponential amplification could be selected for further analysis. Runner! The PCR products were separated on sequencing gels and their fluorescence intensity was analyzed by GeneScan software. Examples of the RT-PCR plots are shown in mannerism Figure 3 . Adult rat brain was found to have the highest ratio of Zoonosis Essay, long/short form ClC-2 transcripts, while the lowest ratios were found in adult and mannerism fetal rat lungs (Table2). This difference between adult brain and all other tissues that we studied here is tess d'urbervilles analysis, statistically significant ( P 0.05, by one way ANOVA, followed by the Bonferroni). The lower long/short ratios in adult and fetal rat lungs are consistent with a similar ratio in the L2 cell line (type II-like rat alveolar pneumocytes) ( Table 2 ). Mannerism! Although the ratios of the two forms were estimated within each tissue and compared between tissues, this experiment did not intend to compare quantitatively the amount of of php, each form alone between tissues.
Our results suggest that ClC-2S is favored relative to ClC-2 in cells of mannerism examples, lung-derivation compared with that of brain. Examples of ii, RT-PCR plot from adult rat tissues and cells. Primers HRC5? and HRC3? were used in the amplification. Number of cycles are labeled. Solid lines are short form products and mannerism dashed lines are long form products. Examples of RT-PCR plot from adult rat tissues and cells.
Primers HRC5? and HRC3? were used in the amplification. Number of cycles are labeled. Solid lines are short form products and dashed lines are long form products. Rat ClC-2 transcript contains a truncated form produced by alternative splicing. A number of ClC channels have been described among which ClC-2 is Depression, ubiquitously expressed in rat, rabbit and mannerism examples human tissues based on Northern hybridization ( 8 , 10 , 11 ). ClC channel protein short forms, however, have been demonstrated only in a few cases. The Canadian! In rat ClC-K2, a 55 amino acid residue fragment in the second membrane spanning domain has been found truncated in mannerism examples a short form, ClC-K2S ( 3 ). In rabbit, a short form of ClC-2G has been reported with a 68 amino acid residue truncation at the very N-terminal end ( 12 ). In both cases, the authors speculated that different RNA processing might be the cause of the truncation. ClC-2 is different from ClC-K2 and ClC-2G in that there is a deleted portion within the cytosolic domain, which is less conserved among ClC channel family members compared with other regions of the protein. This makes non-specific PCR amplification less likely.
The possibility of Infectious Diseases: Zoonosis Essay, non-specific PCR amplification of other ClC channel genes is further eliminated by using three different primer pairs in our experiments, including using the full-length cDNA clone pRK as the PCR template, size determination of the PCR products by GeneScan, and ultimately, cloning and mannerism examples sequencing of the two PCR fragments. In addition to the observed exon skipping in the cytosolic domain, other ClC-2 transcripts in which exon-skipping occurs in other regions of the gene may also exist. We have detected a full length ClC-2 cDNA from rat kidney by RT-PCR differing from the published rat brain sequence by only the 60 bp deletion, indicating the presence of the short form transcript ClC-2S without any other variations. The Kite Devices! The putative amino acid sequence of the deleted region is highly positively charged, and the genomic structure is conserved in rat and human. Both of these may suggest a functional or structural importance of mannerism, this alternative splicing and Infectious possible participation of the short form in the rat ClC-2 regulation or function. When the mannerism, human cell lines 9/HTEo- and NHBE were analyzed by RT-PCR, ClC-2S was not detected. The conservation of the d'urbervilles analysis, upstream intron in human and rat predicts that ClC-2S may be produced in human tissues in vivo . Mannerism! Alternative splicing generates diversity in chloride channel forms by Zoonosis, several different mechanisms. Alternative RNA splicing is one of the mannerism, most common strategies that cells use to generate further diversity ( 21 ). The mechanisms involved in exon skipping are diverse and not well understood. Small exon size itself may facilitate the disadvantages of php, aberrant splicing ( 22 ). However, a number of examples of exon skipping involve changes in some basic elements in their DNA sequences such as branch point sequences, polypyrimidine tracts and 3? splice sites in their upstream introns.
Mutations introduced at these points led to exon skipping, and return of these elements to their respective consensus sequences appeared to correct the mannerism, skipping ( 23 , 24 ). While the structure of these conserved DNA elements is necessary for normal RNA splicing in many cases, only a few studies showed unusual amounts of Depression, pyrimidine residues in the introns upstream from deleted exons. In the mannerism, case of CFTR (cystic fibrosis transmembrane conductance regulator) exon 9, an inverse relationship has been demonstrated between the length of a polythymidine tract in its intron 8 acceptor site and the proportion of exon 9 ? CFTR transcript, i.e., less exon 9 skipping was observed in william henry ii cases with a longer polythymidine tract ( 25 ). High pyrimidine content is also evident in mannerism examples the rat ?-tropomyosin gene intron 6 (68%) where exon 7 is skipped in skeletal muscle ( 26 ). Henry! The high pyrimidine content of the upstream intron in the ClC-2 gene may be one of the basic cis -acting elements which participate in the alternative splicing, however further evidence is needed to confirm its role. Another possible mechanism which could lead to mannerism examples, exon skipping is the failure to william, recognize the 3?-splice site of the upstream intron. Competition between 3?-splice sites for recognition is examples, largely dependent on their structure. AAG in the upstream intron 3?-splice site is unique to Infectious Diseases: Essay, all of the ClC-2 introns sequenced to date (data not shown), others being either TAG or CAG, both preferred for recognition over AAG ( 20 ). The high pyrimidine content and mannerism examples the 3?-splice site sequence may constitute basic regulatory elements present in the rat genomic DNA. Since lung has a lower ratio of long form/short form transcripts than the brain, there might be other trans -acting regulatory factors with different abundance or activity in either lung or brain that interact with the upstream intron or splice sites and either promote the exon skipping as seen in the lung or help retain the exon as seen in disadvantages the brain. ClC-2 and CFTR are both expressed in organs affected in cystic fibrosis. Mannerism Examples! CFTR is a cAMP-regulated chloride channel ( 27 ) expressed in many secretory epithelia.
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Plural Marriage in examples Kirtland and Nauvoo. Latter-day Saints believe that monogamy—the marriage of one man and one woman—is the definition Lord’s standing law of marriage. Mannerism Examples. 1 In biblical times, the Lord commanded some of His people to practice plural marriage—the marriage of the kite runner literary one man and more than one woman. 2 Some early members of The Church of mannerism Jesus Christ of Infectious Zoonosis Essay Latter-day Saints also received and obeyed this commandment given through God’s prophets. After receiving a revelation commanding him to examples practice plural marriage, Joseph Smith married multiple wives and introduced the practice to close associates. This principle was among the most challenging aspects of the Depression Essay Restoration—for Joseph personally and for other Church members. Plural marriage tested faith and mannerism examples provoked controversy and opposition.
Few Latter-day Saints initially welcomed the restoration of a biblical practice entirely foreign to their sensibilities. But many later testified of powerful spiritual experiences that helped them overcome their hesitation and gave them courage to accept this practice. Although the of php Lord commanded the adoption—and later the cessation—of plural marriage in the latter days, He did not give exact instructions on how to obey the commandment. Significant social and cultural changes often include misunderstandings and examples difficulties. Church leaders and members experienced these challenges as they heeded the command to practice plural marriage and again later as they worked to freedom definition discontinue it after Church President Wilford Woodruff issued an examples inspired statement known as the Diseases: Zoonosis Manifesto in 1890, which led to the end of plural marriage in the Church. Through it all, Church leaders and members sought to follow God’s will. Many details about the early practice of plural marriage are unknown. Plural marriage was introduced among the early Saints incrementally, and participants were asked to keep their actions confidential.
They did not discuss their experiences publicly or in writing until after the Latter-day Saints had moved to Utah and mannerism examples Church leaders had publicly acknowledged the william henry gates ii practice. The historical record of early plural marriage is therefore thin: few records of the time provide details, and later reminiscences are not always reliable. Some ambiguity will always accompany our knowledge about examples this issue. Like the participants, we “see through a glass, darkly” and are asked to The Canadian Depression Essay walk by faith. 3. The Beginnings of Plural Marriage in the Church. The revelation on plural marriage was not written down until 1843, but its early verses suggest that part of it emerged from Joseph Smith’s study of the Old Testament in 1831. Mannerism Examples. People who knew Joseph well later stated he received the revelation about disadvantages that time. 4 The revelation, recorded in Doctrine and Covenants 132, states that Joseph prayed to know why God justified Abraham, Isaac, Jacob, Moses, David, and Solomon in having many wives.
The Lord responded that He had commanded them to enter into the practice. 5. Latter-day Saints understood that they were living in examples the latter days, in what the revelations called the “dispensation of the henry gates fulness of times.” 6 Ancient principles—such as prophets, priesthood, and mannerism examples temples—would be restored to definition the earth. Plural marriage was one of those ancient principles. Polygamy had been permitted for millennia in many cultures and religions, but, with few exceptions, was rejected in Western cultures. Mannerism. 7 In Joseph Smith’s time, monogamy was the only legal form of marriage in the United States. Joseph knew the absolute definition practice of plural marriage would stir up public ire.
After receiving the mannerism commandment, he taught a few associates about it, but he did not spread this teaching widely in the 1830s. D'urbervilles Analysis. 8. When God commands a difficult task, He sometimes sends additional messengers to encourage His people to obey. Consistent with this pattern, Joseph told associates that an angel appeared to him three times between 1834 and 1842 and commanded him to proceed with plural marriage when he hesitated to move forward. During the mannerism examples third and disadvantages final appearance, the angel came with a drawn sword, threatening Joseph with destruction unless he went forward and obeyed the commandment fully. 9. Fragmentary evidence suggests that Joseph Smith acted on mannerism the angel’s first command by marrying a plural wife, Fanny Alger, in Kirtland, Ohio, in the mid-1830s.
Several Latter-day Saints who had lived in Kirtland reported decades later that Joseph Smith had married Alger, who lived and worked in the Smith household, after he had obtained her consent and that of her parents. 10 Little is known about this marriage, and nothing is known about the conversations between Joseph and Emma regarding Alger. After the marriage with Alger ended in separation, Joseph seems to have set the tess of the d'urbervilles subject of plural marriage aside until after the Church moved to Nauvoo, Illinois. Plural Marriage and Eternal Marriage. The same revelation that taught of plural marriage was part of a larger revelation given to Joseph Smith—that marriage could last beyond death and that eternal marriage was essential to examples inheriting the fulness that God desires for His children. As early as 1840, Joseph Smith privately taught Apostle Parley P. Disadvantages. Pratt that the “heavenly order” allowed Pratt and his wife to be together “for time and all eternity.” 11 Joseph also taught that men like Pratt—who had remarried following the death of his first wife—could be married (or sealed) to their wives for eternity, under the proper conditions. Mannerism Examples. 12.
The sealing of husband and wife for Infectious Essay, eternity was made possible by the restoration of priesthood keys and ordinances. On April 3, 1836, the Old Testament prophet Elijah appeared to mannerism examples Joseph Smith and Oliver Cowdery in the Kirtland Temple and restored the priesthood keys necessary to perform ordinances for gates ii, the living and the dead, including sealing families together. 13 Marriages performed by priesthood authority could link loved ones to each other for eternity, on mannerism condition of righteousness; marriages performed without this authority would end at death. 14. Marriage performed by priesthood authority meant that the procreation of ii children and perpetuation of families would continue into the eternities. Joseph Smith’s revelation on marriage declared that the “continuation of the mannerism examples seeds forever and ever” helped to The Canadian Depression Essay fulfill God’s purposes for His children.
15 This promise was given to all couples who were married by priesthood authority and were faithful to mannerism their covenants. For much of Western history, family “interest”—economic, political, and social considerations—dominated the choice of spouse. The Kite Runner Literary Devices. Parents had the power to arrange marriages or forestall unions of which they disapproved. By the late 1700s, romance and personal choice began to rival these traditional motives and practices. Mannerism Examples. 16 By Joseph Smith’s time, many couples insisted on marrying for love, as he and Emma did when they eloped against her parents’ wishes. Latter-day Saints’ motives for plural marriage were often more religious than economic or romantic. Henry. Besides the desire to be obedient, a strong incentive was the hope of living in God’s presence with family members. In the revelation on marriage, the Lord promised participants “crowns of eternal lives” and “exaltation in the eternal worlds.” 17 Men and women, parents and children, ancestors and examples progeny were to freedom definition be “sealed” to each other—their commitment lasting into the eternities, consistent with Jesus’s promise that priesthood ordinances performed on earth could be “bound in heaven.” 18. The first plural marriage in mannerism examples Nauvoo took place when Louisa Beaman and Joseph Smith were sealed in April 1841. 19 Joseph married many additional wives and authorized other Latter-day Saints to practice plural marriage. The practice spread slowly at first.
By June 1844, when Joseph died, approximately 29 men and 50 women had entered into absolute freedom, plural marriage, in mannerism addition to Joseph and his wives. When the of php Saints entered the Salt Lake Valley in 1847, at least 196 men and 521 women had entered into plural marriages. 20 Participants in these early plural marriages pledged to examples keep their involvement confidential, though they anticipated a time when the of php practice would be publicly acknowledged. Nevertheless, rumors spread. A few men unscrupulously used these rumors to seduce women to join them in an unauthorized practice sometimes referred to as “spiritual wifery.” When this was discovered, the men were cut off from the Church. 21 The rumors prompted members and leaders to mannerism issue carefully worded denials that denounced spiritual wifery and polygamy but were silent about what Joseph Smith and The Canadian Depression others saw as divinely mandated “celestial” plural marriage. 22 The statements emphasized that the Church practiced no marital law other than monogamy while implicitly leaving open the possibility that individuals, under direction of God’s living prophet, might do so. 23.
During the era in examples which plural marriage was practiced, Latter-day Saints distinguished between sealings for time and eternity and sealings for eternity only. Sealings for time and eternity included commitments and relationships during this life, generally including the possibility of sexual relations. Eternity-only sealings indicated relationships in the next life alone. Evidence indicates that Joseph Smith participated in both types of sealings. The exact number of women to whom he was sealed in his lifetime is unknown because the evidence is fragmentary.
24 Some of the the kite runner devices women who were sealed to Joseph Smith later testified that their marriages were for mannerism, time and eternity, while others indicated that their relationships were for eternity alone. 25. Most of those sealed to Joseph Smith were between 20 and 40 years of age at henry gates ii, the time of their sealing to mannerism examples him. Absolute Freedom. The oldest, Fanny Young, was 56 years old. The youngest was Helen Mar Kimball, daughter of Joseph’s close friends Heber C. and Vilate Murray Kimball, who was sealed to Joseph several months before her 15th birthday. Marriage at such an age, inappropriate by today’s standards, was legal in mannerism that era, and some women married in their mid-teens. Disadvantages. 26 Helen Mar Kimball spoke of examples her sealing to Joseph as being “for eternity alone,” suggesting that the Diseases: Zoonosis relationship did not involve sexual relations.
27 After Joseph’s death, Helen remarried and became an examples articulate defender of him and william of plural marriage. 28. Following his marriage to Louisa Beaman and examples before he married other single women, Joseph Smith was sealed to of the d'urbervilles analysis a number of women who were already married. Examples. 29 Neither these women nor Joseph explained much about Diseases: Zoonosis these sealings, though several women said they were for mannerism, eternity alone. Infectious Diseases: Zoonosis. 30 Other women left no records, making it unknown whether their sealings were for time and eternity or were for eternity alone. There are several possible explanations for this practice.
These sealings may have provided a way to create an eternal bond or link between Joseph’s family and mannerism other families within the Church. 31 These ties extended both vertically, from parent to child, and horizontally, from one family to another. Today such eternal bonds are achieved through the disadvantages of php temple marriages of mannerism examples individuals who are also sealed to their own birth families, in this way linking families together. Joseph Smith’s sealings to of the women already married may have been an early version of mannerism examples linking one family to another. In Nauvoo, most if not all of the first husbands seem to have continued living in the same household with their wives during Joseph’s lifetime, and complaints about these sealings with Joseph Smith are virtually absent from the documentary record. 32. These sealings may also be explained by Joseph’s reluctance to enter plural marriage because of the sorrow it would bring to his wife Emma. He may have believed that sealings to married women would comply with the Lord’s command without requiring him to have normal marriage relationships. Of The D'urbervilles Analysis. 33 This could explain why, according to examples Lorenzo Snow, the angel reprimanded Joseph for the kite devices, having “demurred” on mannerism examples plural marriage even after he had entered into the practice. 34 After this rebuke, according to this interpretation, Joseph returned primarily to sealings with single women. Another possibility is that, in an era when life spans were shorter than they are today, faithful women felt an urgency to freedom be sealed by priesthood authority.
Several of these women were married either to non-Mormons or former Mormons, and more than one of the women later expressed unhappiness in their present marriages. Living in a time when divorce was difficult to obtain, these women may have believed a sealing to Joseph Smith would give them blessings they might not otherwise receive in examples the next life. 35. The women who united with Joseph Smith in plural marriage risked reputation and self-respect in being associated with a principle so foreign to disadvantages their culture and so easily misunderstood by others. “I made a greater sacrifice than to give my life,” said Zina Huntington Jacobs, “for I never anticipated again to be looked upon mannerism, as an honorable woman.” Nevertheless, she wrote, “I searched the scripture by humble prayer to my Heavenly Father I obtained a testimony for myself.” 36 After Joseph’s death, most of the women sealed to him moved to Utah with the Saints, remained faithful Church members, and defended both plural marriage and Joseph. 37. Plural marriage was difficult for all involved. For Joseph Smith’s wife Emma, it was an excruciating ordeal. Records of william henry ii Emma’s reactions to plural marriage are sparse; she left no firsthand accounts, making it impossible to reconstruct her thoughts.
Joseph and Emma loved and respected each other deeply. Examples. After he had entered into plural marriage, he poured out his feelings in the kite runner literary his journal for mannerism examples, his “beloved Emma,” whom he described as “undaunted, firm and unwavering, unchangeable, affectionate Emma.” After Joseph’s death, Emma kept a lock of absolute freedom definition his hair in a locket she wore around her neck. 38. Emma approved, at mannerism, least for a time, of four of Joseph Smith’s plural marriages in disadvantages Nauvoo, and she accepted all four of those wives into her household. She may have approved of other marriages as well. 39 But Emma likely did not know about all of Joseph’s sealings. Examples. 40 She vacillated in her view of plural marriage, at some points supporting it and at other times denouncing it. In the disadvantages summer of 1843, Joseph Smith dictated the revelation on mannerism marriage, a lengthy and complex text containing both glorious promises and stern warnings, some directed at Emma. 41 The revelation instructed women and men that they must obey God’s law and commands in order to henry gates receive the examples fulness of henry His glory. The revelation on marriage required that a wife give her consent before her husband could enter into plural marriage.
42 Nevertheless, toward the end of the revelation, the Lord said that if the first wife “receive not this law”—the command to practice plural marriage—the husband would be “exempt from the law of Sarah,” presumably the requirement that the husband gain the consent of the examples first wife before marrying additional women. 43 After Emma opposed plural marriage, Joseph was placed in an agonizing dilemma, forced to choose between the will of God and the will of his beloved Emma. He may have thought Emma’s rejection of plural marriage exempted him from the absolute law of Sarah. Her decision to mannerism examples “receive not this law” permitted him to marry additional wives without her consent. Because of devices Joseph’s early death and Emma’s decision to mannerism remain in Nauvoo and not discuss plural marriage after the Church moved west, many aspects of their story remain known only to the two of them. Years later in henry ii Utah, participants in Nauvoo plural marriage discussed their motives for entering into the practice. Mannerism. God declared in the Book of Mormon that monogamy was the standard; at times, however, He commanded plural marriage so His people could “raise up seed unto [Him].” 44 Plural marriage did result in freedom definition an increased number of children born to believing parents.
45. Some Saints also saw plural marriage as a redemptive process of sacrifice and spiritual refinement. Mannerism. According to absolute definition Helen Mar Kimball, Joseph Smith stated that “the practice of this principle would be the mannerism hardest trial the Saints would ever have to test their faith.” Though it was one of the “severest” trials of her life, she testified that it had also been “one of the greatest blessings.” 46 Her father, Heber C. Kimball, agreed. “I never felt more sorrowful,” he said of the the kite runner devices moment he learned of plural marriage in 1841. “I wept days. … I had a good wife. I was satisfied.” 47. The decision to accept such a wrenching trial usually came only after earnest prayer and intense soul-searching. Brigham Young said that, upon learning of plural marriage, “it was the first time in my life that I had desired the grave.” 48 “I had to mannerism examples pray unceasingly,” he said, “and I had to exercise faith and the Lord revealed to me the truth of of the d'urbervilles it and that satisfied me.” 49 Heber C. Kimball found comfort only after his wife Vilate had a visionary experience attesting to the rightness of plural marriage. “She told me,” Vilate’s daughter later recalled, “she never saw so happy a man as father was when she described the vision and told him she was satisfied and knew it was from God.” 50. Lucy Walker recalled her inner turmoil when Joseph Smith invited her to become his wife. “Every feeling of my soul revolted against it,” she wrote. Yet, after several restless nights on her knees in prayer, she found relief as her room “filled with a holy influence” akin to “brilliant sunshine.” She said, “My soul was filled with a calm sweet peace that I never knew,” and “supreme happiness took possession of my whole being.” 51. Not all had such experiences. Some Latter-day Saints rejected the principle of plural marriage and mannerism left the Church, while others declined to enter the Diseases: Essay practice but remained faithful.
52 Nevertheless, for many women and men, initial revulsion and anguish was followed by struggle, resolution, and ultimately, light and peace. Mannerism. Sacred experiences enabled the Saints to Infectious Zoonosis Essay move forward in faith. Mannerism. 53. The challenge of introducing a principle as controversial as plural marriage is disadvantages of php, almost impossible to overstate. A spiritual witness of its truthfulness allowed Joseph Smith and other Latter-day Saints to accept this principle.
Difficult as it was, the mannerism examples introduction of plural marriage in Nauvoo did indeed “raise up seed” unto God. A substantial number of today’s members descend through faithful Latter-day Saints who practiced plural marriage. Church members no longer practice plural marriage. 54 Consistent with Joseph Smith’s teachings, the Church permits a man whose wife has died to be sealed to another woman when he remarries. Moreover, members are permitted to Infectious Zoonosis Essay perform ordinances on behalf of deceased men and women who married more than once on earth, sealing them to all of the spouses to whom they were legally married. The precise nature of these relationships in the next life is not known, and many family relationships will be sorted out in the life to examples come. Latter-day Saints are encouraged to trust in our wise Heavenly Father, who loves His children and does all things for literary devices, their growth and salvation. 55.
See “The Family: A Proclamation to the World”; Jacob 2:27, 30. Mannerism Examples. Doctrine and absolute freedom Covenants 132:34–39; Jacob 2:30; see also Genesis 16. 1 Corinthians 13:12; Jeffrey R. Holland, “Lord, I Believe,” Ensign, May 2013. See Andrew Jenson, “Plural Marriage,” Historical Record 6 (May 1887): 232–33; “Report of Elders Orson Pratt and Joseph F. Smith,” Millennial Star 40 (Dec. 16, 1878): 788; Danel W. Mannerism Examples. Bachman, “New Light on an Old Hypothesis: The Ohio Origins of the Revelation on Eternal Marriage,” Journal of Mormon History 5 (1978): 19–32. Devices. See Doctrine and Covenants 132:1, 34–38. Doctrine and Covenants 112:30; 124:41; 128:18. Mannerism Examples. “Polygamy,” in The Oxford Dictionary of World Religions, ed. John Bowker (New York: Oxford University Press, 1997), 757; John Cairncross, After Polygamy Was Made a Sin: The Social History of Christian Polygamy (London: Routledge Kegan Paul, 1974). Lorenzo Snow, deposition, United States Testimony 1892 (Temple Lot Case), part 3, p. 124, Church History Library, Salt Lake City; Orson Pratt, in Journal of Discourses, 13:193; Ezra Booth to Ira Eddy, Dec.
6, 1831, in Ohio Star, Dec. 8, 1831. See Brian C. Hales, “Encouraging Joseph Smith to Practice Plural Marriage: The Accounts of the Angel with a Drawn Sword,” Mormon Historical Studies 11, no. William Henry Gates Ii. 2 (Fall 2010): 69–70. See Andrew Jenson, Research Notes, Andrew Jenson Collection, Church History Library, Salt Lake City; Benjamin F. Johnson to Gibbs, 1903, Benjamin F. Johnson Papers, Church History Library, Salt Lake City; “Autobiography of Levi Ward Hancock,” Church History Library, Salt Lake City. Mannerism. Parley P. Pratt, The Autobiography of Parley Parker Pratt, One of the Twelve Apostles of The Church of Jesus Christ of Latter-day Saints, ed. Depression Essay. Parley P. Pratt Jr. (New York: Russell Brothers, 1874), 329. Hyrum Smith, sermon, Apr. 8, 1844, Historian’s Office General Church Minutes, Church History Library, Salt Lake City.
These were the examples same priesthood keys Elijah had given to Apostles anciently. Absolute. (See Matthew 16:19; 17:1–9; Doctrine and Covenants 2.) Doctrine and mannerism examples Covenants 132:7; 131:2–3. Doctrine and d'urbervilles analysis Covenants 132:19–20, 63; see also “Becoming Like God.” Stephanie Coontz, Marriage, A History: From Obedience to Intimacy, or How Love Conquered Marriage (New York: Viking Penguin, 2005), 145–60; Lawrence Stone, The Family, Sex and Marriage in England, 1500–1800, abridged ed. (Middlesex, UK: Penguin Books, 1985), 217–53. Doctrine and Covenants 132:55, 63. Doctrine and Covenants 132:46; Matthew 16:19. Joseph Smith’s practice of plural marriage has been discussed by examples, Latter-day Saint authors in official, semi-official, and independent publications.
See, for tess analysis, example, Jenson, “Plural Marriage,” 219–34; B. H. Roberts, A Comprehensive History of The Church of Jesus Christ of Latter-day Saints, 6 vols. (Salt Lake City: Deseret News Press, 1930), 2:93–110, Danel W. Examples. Bachman and Diseases: Zoonosis Essay Ronald K. Esplin, “Plural Marriage,” in examples Encyclopedia of Mormonism, 4 vols. (New York: Macmillan, 1992), 2:1091-95; and Glen M. Leonard, Nauvoo: A Place of Peace, a People of Promise (Salt Lake City and Provo, UT: Deseret Book and Brigham Young University, 2002), 343–49. Brian C. Hales, Joseph Smith’s Polygamy, 3 vols. (Salt Lake City: Greg Kofford Books, 2013) , 1:3, 2:165. Joseph Smith, Journal, May 19, 24, and absolute freedom 26, 1842; June 4, 1842, available at josephsmithpapers.org. Proponents of “spiritual wifery” taught that sexual relations were permissible outside of legalized marital relationships, on condition that the relations remained secret. In the denials, “polygamy” was understood to mannerism examples mean the marriage of one man to more than one woman but without Church sanction. See, for example, “On Marriage,” Times and the kite devices Seasons, Oct. 1, 1842, 939–40; and Wilford Woodruff journal, Nov. 25, 1843, Church History Library, Salt Lake City; Parley P. Pratt, “This Number Closes the First Volume of the ‘Prophet,’” The Prophet, May 24, 1845, 2. Examples. George A. Smith explained, “Any one who will read carefully the denials, as they are termed, of plurality of Infectious wives in mannerism examples connection with the william circumstances will see clearly that they denounce adultery, fornication, brutal lust and the teaching of plurality of wives by those who were not commanded to do so” (George A. Smith letter to Joseph Smith III, Oct. 9, 1869, in Journal History of The Church of Jesus Christ of Latter-day Saints, Oct. 9, 1869, Church History Library, Salt Lake City).
Careful estimates put the number between 30 and 40. See Hales, Joseph Smith’s Polygamy, 2:272–73. Mannerism Examples. See Hales, Joseph Smith’s Polygamy , 2:277–302. Despite claims that Joseph Smith fathered children within plural marriage, genetic testing has so far been negative, though it is possible he fathered two or three children with plural wives. (See Ugo A. Perego, “Joseph Smith, the Question of Essay Polygamous Offspring, and mannerism examples DNA Analysis,” in Newell G. Bringhurst and Craig L. Foster, eds., The Persistence of Polygamy: Joseph Smith and the kite runner devices the Origins of Mormon Polygamy [Independence, MO: John Whitmer Books, 2010], 233–56.) J. Spencer Fluhman, “A Subject that Can Bear Investigation’: Anguish, Faith, and Joseph Smith’s Youngest Plural Wife,” in Robert L. Millet, ed., No Weapon Shall Prosper: New Light on Sensitive Issues (Provo and Salt Lake City: Brigham Young University Religious Studies Center and Deseret Book, 2011), 104–19; Craig L. Foster, David Keller, and examples Gregory L. Smith, “The Age of Joseph Smith’s Plural Wives in Social and disadvantages of php Demographic Context,” in Bringhurst and Foster, eds., The Persistence of Polygamy , 152–83. Helen Mar Kimball Whitney, Autobiography, , Church History Library, Salt Lake City. Helen Mar Kimball Whitney, Plural Marriage as Taught by mannerism examples, the Prophet Joseph: A Reply to Joseph Smith, Editor of the Lamoni (Iowa) “Herald” (Salt Lake City: Juvenile Instructor Office, 1882); Helen Mar Kimball Whitney, Why We Practice Plural Marriage (Salt Lake City: Juvenile Instructor Office, 1884). Estimates of the number of these sealings range from 12 to 14. Disadvantages Of Php. (See Todd Compton, In Sacred Loneliness: The Plural Wives of Joseph Smith [Salt Lake City: Signature Books, 1997], 4, 6; Hales, Joseph Smith’s Polygamy, 1:253–76, 303–48.) For an early summary of this practice, see John A. Widtsoe, “Evidences and mannerism Reconciliations: Did Joseph Smith Introduce Plural Marriage?” Improvement Era 49, no. 11 (Nov. 1946): 766–67. Hales, Joseph Smith’s Polygamy, 1:421–37.
Polyandry, the marriage of one woman to more than one man, typically involves shared financial, residential, and sexual resources, and children are often raised communally. There is no evidence that Joseph Smith’s sealings functioned in this way, and much evidence works against definition that view. Rex Eugene Cooper, Promises Made to the Fathers: Mormon Covenant Organization (Salt Lake City: University of mannerism Utah Press, 1990), 138–45; Jonathan A. Stapley, “Adoptive Sealing Ritual in Mormonism,” Journal of Mormon History 37, no. 3 (Summer 2011): 53–117. For a review of the disadvantages of php evidence, see Hales, Joseph Smith’s Polygamy, 1:390–96. Richard Lyman Bushman, Joseph Smith: Rough Stone Rolling (New York: Knopf, 2005), 440.
See Lorenzo Snow, deposition, United States Testimony 1892 (Temple Lot Case), part 3, p. 124. The revelation on marriage provided powerful incentives for a marriage performed by priesthood authority. Examples. (See Doctrine and Covenants 132:17–19, 63.) Zina Huntington Jacobs, autobiographical sketch, Zina Card Brown Family Collection, Church History Library, Salt Lake City; spelling modernized. The historical record is henry gates, striking for the lack of mannerism criticism found among those who had once been Joseph Smith’s plural wives, although most of the wives left no written record. Joseph Smith, Journal, Aug. 16, 1842, in Andrew H. Hedges, Alex D. Disadvantages. Smith, and Richard Lloyd Anderson, eds., Journals, Volume 2: December 1841–April 1843 , vol. 2 of the Journals series of The Joseph Smith Papers , edited by Dean C. Jessee, Ronald K. Esplin, and Richard Lyman Bushman (Salt Lake City: Church Historian’s Press, 2011), 93–96, available at josephsmithpapers.org; Mary Audentia Smith Anderson, ed ., Joseph Smith III and the Restoration (Independence, MO: Herald House, 1952), 85. Jenson, “Historical Record,” 229–30, 240; Emily Dow Partridge Young, deposition, United States Testimony 1892 (Temple Lot Case), part 3, pp. 365–66, 384; Orson Pratt, in Journal of Discourses, 13:194. Hales, Joseph Smith’s Polygamy, 2:8, 48–50, 80; Bushman, Rough Stone Rolling, 473.
Doctrine and Covenants 132:54, 64. The warning to Emma Smith also applies to all who receive sacred ordinances by authority of the priesthood but do not abide the covenants associated with those ordinances. See, for example, Psalm 37:38; Isaiah 1:28; Acts 3:19–25; and Doctrine and Covenants 132:26, 64. Mannerism Examples. Doctrine and Covenants 132:61. Absolute Freedom. In Utah, the first wife was part of the plural marriage ceremony, standing between her husband and the bride and placing the hand of the bride in the hand of the husband. “Celestial Marriage,” The Seer 1 (Feb. 1853): 31. Doctrine and Covenants 132:65; see also Genesis 16:1–3.
Jacob 2:30. On the question of children, see note 6 of “Plural Marriage and Families in Early Utah.” Helen Mar Kimball Whitney, Why We Practice Plural Marriage, 23–24. Heber C. Examples. Kimball, Discourse, Sept. 2, 1866, George D. Watt Papers, Church History Library, Salt Lake City, transcribed from gates, Pitman shorthand by LaJean Purcell Carruth. Brigham Young, in Journal of Discourses, 3:266. Brigham Young, Discourse, June 18, 1865, George D. Watt Papers, Church History Library, Salt Lake City, transcribed from Pitman shorthand by mannerism examples, LaJean Purcell Carruth; see also Brigham Young, in Journal of Discourses, 11:128.
Orson F. Whitney, Life of Heber C. Kimball, an Apostle: The Father and Founder of the British Mission (Salt Lake City: Kimball Family, 1888), 338; see also Kiersten Olson, “‘The Embodiment of Strength and Endurance’: Vilate Murray Kimball (1806–1867),” in ii Women of mannerism examples Faith in the Latter Days, Volume One, 1775–1820, ed. Richard E. Turley Jr. and absolute definition Brittany A. Chapman (Salt Lake City: Deseret Book, 2011), 137. Lucy Walker Kimball, “Brief Biographical Sketch,” 10–11, Church History Library, Salt Lake City. Sarah Granger Kimball, for examples, example, rejected plural marriage in Nauvoo but came west with the Saints. Many of the individuals who rejected plural marriage, including Emma Smith, later became members of the the kite devices Reorganized Church of Jesus Christ of Latter Day Saints.
For example, see “Evidence from Zina D. Huntington-Young,” Saints’ Herald, Jan. Mannerism Examples. 11, 1905, 29; Mary Elizabeth Rollins Lightner, “Mary Elizabeth Rollins,” Susa Young Gates Papers, Utah State Historical Society. Gordon B. Of Php. Hinckley, “What Are People Asking about Us?” Ensign, Nov. 1998; “Polygamy,” Newsroom, topics page. Examples. Alma 26:35; Doctrine and Covenants 88:41; 1 Nephi 11:17.
The Church acknowledges the contribution of scholars to the historical content presented in this article; their work is used with permission.
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In many organizations, workers now see the mannerism outside of HR as. Frankenstein and The Monster Description Essay. Frankenstein’s thirst for knowledge led him to venture on Infectious, to be a student at the University of Ingolstadt to major in mannerism, science. There, Victor learned and Infectious Diseases:, mastered natural philosophy. His professor, Dr. Waldman, played a major role in Frankenstein’s philosophy. Dr. Waldman introduced Victor to the idea of using electricity to regenerate tissue. Those ideas become Frankenstein’s life for the time to follow.
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Furthermore nothing has been more divisive to cultures around the world than religion. Religion is the one. Being able to take direction from someone pointing out our mistakes and then bettering ourselves, this would be a huge skill set that many employers would greatly covet. 4) Holding yourself accountable: This is a skill set that many people find extremely difficult to manage. Nobody likes to be wrong.
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Job Description Channel Management Essay. MAJOR CHALLENGES * Motivate and incentive channels to reach ambitious targets * Be always Innovative to get newer ways to optimise sales in Channels * Regular Market Visit is tess of the analysis a Must : to get real scenario on Deep Dive * Essay Analysis and Description of Aquatic Therapy. Styles and Versions Aquatic therapy can be performed in examples, various styles and versions. Different techniques and the kite runner literary, treatment forms can be used to achieve a better quality of life for individuals. Mannerism Examples. Some of these styles and versions include: - Hydrotherapy: is the application of water to initiate cure. All three forms of of php water (liquid, steam, ice) can be used therapeutically. The goal is to improve circulation and the quality of blood (Health Communities, 2014). - Whirlpools: refers to mannerism examples, a special kind. Essay on Mechanism Description of an Electric Guitar. The neck is fixed to the guitar’s body, and can be glued or bolted at Depression Essay, the neck joint (2.3). The Body The body is the bottom part of the guitar. It can be made of mannerism examples wood, plastic or cardboard, and its shape and size depend on the manufacturers’ style: the average body is 14 inch wide, 21 inch long and 1.75 inch thick.
The body of an ii electric guitar has many functions due to the large number of examples subparts it contains. First of all, we have the tailpiece (3.4), a component that holds the opposite. Essay about Description of a Court Room Hearing. Also to change the probation order to keep the runner devices accused from causing the victim fear when the accused was in, or near, a social gathering or building that the victim was present. The victim was not happy with the order on how it stood. The victim had asked for the order to read that the mannerism accused attain from buildings or social gatherings that the definition victim may be in at the time. Mannerism Examples. The victim’s argument was that the of php accused person was stalking her in ways that he could get away with legally and that. A Description of the Business of Medsaic Pty Ltd. Mannerism. Essay. The first DotScanTM product to enter the market was for the diagnosis of leukaemia and runner literary, lymphoma applications in mid-2005.
The market for the product is valued at more than $60 million in Australia and $1.2 billion in the United States of America. The current benchmark protocol for mannerism examples, leukaemia and lymphoma diagnosis is Flow Cytometry, which allows for the identification of. 4-5 markers on the cell surface. In contrast, DotScanTM technology is able to identify anywhere up to 200 markers. While the. Case Description and Theoretical Analysis of Tracy Essay.
During Tracy’s friendship with Evie, the runner majority of mannerism her “support” primarily came from her. The support provided was limited to william henry gates, the encouragement to behave in a way that Evie found beneficial to her. Tracy’s initial decision to steal was because of Evie’s actions and mannerism, encouragement. Also, the gates additional occurrences of stealing, ignoring her previous friends, lying to her mother and so on were due to Evie’s influence. Mental Status Exam Tracy’s appearance has evolved through the observed four months.
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Free High School Student Resume Templates for Teens. If you’ve never made a resume before, it can seem more difficult than the job search itself. Fortunately, making a resume is as simple as following a format. Examples! With some key information in the right order, anyone can create one. Your First Job Resume: What It Is and Why You Need It. A resume is the kite runner devices, a one-page document that summarizes your experience, skills, and other information . The purpose is to show you’re a perfect candidate for the position you are applying for. It should be formal, professional, and examples relevant. Resumes give potential employers a way to the kite devices learn about mannerism, applicants quickly and easily, and they are your first step toward new job opportunities. Runner Devices! An interview might also be needed to mannerism examples land the job, but a resume is necessary to start the gates, process. Resumes let employers quickly screen out examples job seekers who don’t have the right experience and gates ii qualifications. A well-crafted resume that highlights your experience will set you apart from the mannerism examples, competition.
Even if you have little or no work experience yet, these guidelines will get you on track to having a great entry-level resume and all the opportunities that come with it. There are three basic resume formats to choose from: chronological, functional, and combination. It helps to familiarize yourself with these options before deciding on the right resume template for you. This is one of the most common resume formats. Disadvantages! It lists your work history in reverse chronological order, starting with the most recent position. It is examples, often headed with a “Career Objective” section.
We will talk about Diseases: Zoonosis, these sections in more detail later in this post. On a chronological resume you’ll also want to list your education in reverse chronological order, starting with the most recent school you attended or relevant course you’ve taken. This is a common resume format. It’s easy for employers to scan a chronological resume quickly and get an idea of your experience. This kind of resume highlights your work history, so it’s great if you’ve had impressive employment opportunities. If you don’t have much work history, or your work history isn’t relevant to the job you want, this type of resume may not be best for you. Prospective employers will throw your resume in the “No” pile if all they see is work that’s not related to mannerism the job they are looking to fill, or if your resume looks empty because you didn’t have much experience to the kite devices list. A functional resume format highlights other sections, like skills and activities, over work experience. Examples! This type of absolute freedom, resume focuses on what you know or what you can do, rather than what you’ve done at mannerism examples specific jobs.
It will probably still have some sort of work history section, but it will be placed at devices the bottom rather than at the top of the page. Your work history might not be listed in chronological order on this type of resume. Mannerism! In fact, it might not have dates at all, but be listed in gates, order of examples, relevance instead. This type of d'urbervilles analysis, resume can be great if you have little or no conventional work experience. It can hide long gaps in between jobs, and can draw attention to your other skills or activities.
Most employers will be accustomed to the chronological resume format. Examples! They will likely know that the reason you chose this format is devices, because you have less work experience or less consistent job history than other job seekers. Mannerism! But that doesn’t necessarily mean you’re a less desirable candidate. Like a functional resume, a combination resume puts your skills and of php activities first and foremost. However, this type of resume also lists your work experience in mannerism, reverse chronological order. It just doesn’t put it near the top like a chronological resume does. This hybrid style gives a balance between promoting your skills and your work history. For teens with work experience that’s impressive but short, this kind of resume is the kite runner devices, ideal. You can show off your work history, but also fill in more space by talking about your skills and other knowledge you have. It’s also useful if the examples, work history you have doesn’t quite fit with the job you’re looking for. With a combination resume, you can put the Depression Essay, skills that are relevant to your ideal job first, but still give details about your past work experience.
Making a combination resume can feel overwhelming, since it uses so much information. Be careful to use only the most relevant information so it doesn’t become cluttered or too wordy. How to Create a Good High School Student Resume. Your resume always needs to include your name and mannerism examples contact information. Freedom! List your name, phone number, email address, and city at the top of mannerism, your resume. The Kite Runner Literary Devices! You don’t need to mannerism include your full street address, but you can if you’d like. Everything else can be adjusted to disadvantages of php fit your unique experience.
For example, your resume could include any of the following: Work Experience or Work History Education Career Objective or Summary Professional Skills or Other Skills Activities Hobbies and Interests Achievements, Awards, and Honors References. Each of examples, these sections will consist of absolute freedom, a heading in bold or slightly larger font, followed by mannerism details about that section. For example, under the “Education” heading, you might list the schools you’ve attended and the subjects you focused on. Remember, you probably won’t use all of these headings. Just pick the disadvantages, ones that are most relevant to mannerism examples you and Diseases: Zoonosis the job you’re seeking. Every section requires a slightly different approach. Let’s break it down and examples see how it works. This can be a difficult section to tackle on a teen resume, since you might not have any work experience yet.
Don’t worry though. Everyone was in the same situation when they were looking for their first job! If you have no work experience, you can leave this section out and focus on the other sections. If you do have official work experience, it’s important to mention it in this section, especially if it is henry gates, a job similar to the one you are applying for. Even if you weren’t officially employed, you probably have more work experience to put on your resume than you think.
Part-time work like babysitting, lawn mowing, tutoring, and even volunteer experience or community service all count as good examples of work experience on a teen resume. Under the “Work Experience” heading, list the name of the mannerism examples, company you worked for, your job title, and dates of employment. You should also list your responsibilities, duties, and accomplishments. Depression! That way, it’s clear what kind of examples, experience you gained. Use two or three bullet points to list your responsibilities and duties for the kite devices, each job. See our sample high school student resume templates below for real-world examples of work experience sections. In the “Education” section, state the high school you attended or presently attend and the years you were there or the date you expect to graduate. For example: Crescent High School – Arlington, Virginia. Expected graduation date: May 2017.
If there are classes you’ve taken that are relevant to the job you want, you can list that relevant coursework here. You can mention your future education plans too. For example: I have been accepted to Chicago State University, and I am planning to major in business law I will be taking my AP classes next month to prepare for mannerism, college I will begin attending Chicago State University this fall. The “Career Objective” section shows what kind of job you’re looking for. Tess Analysis! It consists of one or two sentences near the mannerism, top of your resume that describe your desired job. Essay! For example: Offer excellent hostess service in a busy local restaurant Apply my computer skills to practical work Provide quality delivery for local food business.
You can also extend your objective statement into a summary. A summary is a short paragraph that summarizes your experience and mannerism examples key skills. For example: I am an energetic, enthusiastic, and gates active individual with a strong knowledge of grocery items and examples their use by devices people from different economic and social backgrounds. I have a business mindset and am willing to apply these skills as a grocery store employee. This section might seem unnecessary, but it can actually be very helpful to potential employers.
It gives you a chance to describe yourself and your goals in mannerism examples, your own words. It can also be useful when you don’t have much concrete work experience, but do have skills that potential employers should know about. Make sure the disadvantages of php, objective or summary is examples, short, clear, and professional. Professional Skills or Other Skills. You likely have skills that could be relevant to a particular position you are applying for.
These can be listed under “Professional Skills.” Make sure the skills are relevant to the job you want. William Henry Ii! If you are applying for mannerism examples, a gardener job, no need to tess of the mention “Ability to handle cash register”, since that skill doesn’t apply to that job. But you might mention, “Ability to mannerism use lawn mower and freedom definition gardening tools safely.” If you have no work experience, this section can help demonstrate whether you are a good fit for the position. You can also list skills that come from your school or hobbies under “Other Skills.” Make sure they’re still related in some way to what potential employers are looking for. What are some key skills you might list? Creativity Leadership Adaptability Flexibility Positivity Problem-solving Communication Independence Self-motivation Ability to work under pressure. This section gives an employer insight into your character and mannerism examples interests. It might even tie into the “Skills” section. Think of extracurricular activities, sports, and clubs you have participated in.
Taking a lead role in a school play? You are probably creative. Playing football? You might be a good team player. The Canadian Essay! These are qualities that employers are looking for. Some examples of examples, school activities that could look good on a resume include: Sports : playing sports in runner literary devices, a association or for fun, outdoor activities, fitness training, taking sport lessons, coaching. Culture : creating art works, shows, plays, films, games, singing or dancing, DIY projects, creative workshops. Examples! Community : raising donations for various organizations or humanitarian causes, organizing events, helping kids, seniors, animals, or the environment, promoting community enhancements. Just like with skills, only list relevant activities here.
Write down the runner literary devices, activity, dates, and your role/position. Mannerism Examples! For example: West River Hospital, volunteer, summer 2012 Crescent High School Soccer Team, captain, 2005 – 2013 Stage Drama Festival, lead role, summer 2011. If your resume is The Canadian Depression, short on work experience, you can list more details about your activities to show what you gained from them. Examples! You might use bullet points to write what your responsibilities were or what skills you used in disadvantages of php, these activities. This section is similar to activities, as it tells an examples employer more about your character.
Simply make a list of hobbies that might be of interest to potential employers. For example: Reading books Playing tennis Painting and sculpture. It’s great to mention major achievements and awards you’ve earned at school or in your activities. Prospective employers like to see accomplishments that show commitment and the kite runner hard work. For example: Student of the Month, March 2013 Perfect Attendance Award, September 2012 Honor Roll, fall 2012. A reference should be someone who can vouch for your knowledge and skills, or who can confirm your work experience. Your teachers, coaches, or former employers can be good references. Listing your friends or family as references is not recommended, but can be acceptable if you worked for them. Remember that you must ask a person to serve as a reference before you can list them as one.
Many people simply write “References Available on Request” at the bottom of their resumes. Examples! If you do this, make sure you actually have those references available in disadvantages, case a prospective employer requests them. However, if you have limited experience to put on mannerism, your resume, you might want to list the names and contact information of absolute freedom, your references on your resume. Free High School Student Resume Examples. These teen resume samples will make getting started easy. There are general purpose high school student resume templates, as well as resumes for specific work experience. Mannerism! These samples will guide you with a professional resume format and a basic idea of Infectious Diseases: Zoonosis Essay, what to examples write.
We also have High School Graduate Resumes and other professional resume templates. Creative babysitter resume sample with a summary, education, related and The Canadian Essay personal skills, hobbies, and mannerism examples references examples. Size: 183 B Downloads: 6267 Filename: babysitter-cv-template-sample.docx Uploaded: 2013-07-04 06:21:00 / 3136. Generic design with sample content (skills, knowledge) for a position in of the, a fast food industry. Size: 183 B Downloads: 7435 Filename: fast-food-employee-cv.docx Uploaded: 2013-07-04 06:34:00 / 3138. Basic design and common content for examples, any type of position.
Size: 183 B Downloads: 27336 Filename: geneal-purpose-high-school-resume.docx Uploaded: 2013-07-04 06:36:00 / 3140. Another multi-purpose sample, with blue header theme. Includes customer work and volunteer experience. Size: 183 B Downloads: 18024 Filename: teenager-cv-sample.docx Uploaded: 2013-07-04 06:38:00 / 3142. Sample for position in gardening or landscape. Depression! Creative design theme to examples attract attention. Size: 183 B Downloads: 3046 Filename: lawn-care-gardening-job-application.docx Uploaded: 2013-07-04 06:39:00 / 3144.
Resume sample for students who would like to absolute definition promote tutoring services to other students or student#039;s parents. Size: 183 B Downloads: 4214 Filename: student-tutor-cv-sample.docx Uploaded: 2013-07-04 06:41:00 / 3146. Generic resume sample for mannerism examples, part-time grocery store job. Can be easily modified for similar positions in different stores. Size: 183 B Downloads: 6589 Filename: grocery-store-application.docx Uploaded: 2013-07-04 06:42:00 / 3148. Elegant resume for of php, server or host position in a restaurant. Can also be used for examples, kitchen help, cook help, busser, etc. Size: 183 B Downloads: 4308 Filename: waitress-or-hostess-job.docx Uploaded: 2013-07-04 06:44:00 / 3150. Sample teen resume for The Canadian Depression, full-time or part-time volunteer job for a candidate with previous volunteer work. Size: 183 B Downloads: 4673 Filename: volunteer-position-application.docx Uploaded: 2013-07-04 06:45:00 / 3152.
Sample resume for examples, part-time position in warehouse. Size: 183 B Downloads: 5313 Filename: warehouse-position.docx Uploaded: 2013-07-04 06:47:00 / 3154. Sample resume for an apprentice welder and tess of the d'urbervilles analysis assisting in different welding projects. Size: 183 B Downloads: 3777 Filename: welder-helper-job.docx Uploaded: 2013-07-04 06:48:00 / 3156. Professional sample for mannerism examples, a food delivery position.
Can be easily modified for a different position in the food industry. Size: 183 B Downloads: 26440 Filename: food-industry-position.docx Uploaded: 2013-07-04 06:17:00 / 3134. 855.213.0348 | MON-FRI 8A-8P, SAT 8A-5P, SUN 10A-6P CST. 2017 Bold Limited. All rights reserved.
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